Protein engineering, the prerequisite for the advent of all protein scaffolds, remains the driving force in this process. Notably, these are also the classes where members have progressed to clinical trials.Įngineered protein display scaffolds, when specifically selected, can bind virtually to any target. By now, reports on the utilization of the engineered protein scaffolds have expanded to X-ray structure determination of binder/target complexes, imaging of cell surface markers and conjugation with drug payloads. The modular structure of these protein scaffolds and their favorable biophysical & chemical properties, such as small in size, high permeability through the cell membrane, lack of disulfide bonds within the molecule, high thermodynamic stability and lack of aggregation, not only allow the binding of a diverse set of targets in a specific manner and with high affinity, but also suggest the utilization of the rigid protein framework for generic engineering concepts, intracellular applications, for instance. Some scaffolds have focused on broadening their fields of application, while others have occupied their respective niche. Possibilities of engineered protein scaffolds have been explored in research, diagnostics and therapy in the last few years. Creative Biolabs has developed trimer codon technology, which offers site-directed mutant library construction in a high-throughput and extremely precise way.Įngineered protein scaffolds are invaluable tools for a vast range of biotechnological and pharmaceutical applications. Our service has provided a strong foundation for the successful isolation and exploitation of high-affinity binders in the fields of research, diagnosis, and therapy. HighlightsĮngineered Protein Scaffold Library Construction ServiceĬreative Biolabs offers engineered protein scaffold library construction service. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects. Cell-based Membrane Protein Expression and PurificationĬreative Biolabs is offering the most comprehensive services for antibody development projects.Cell-free Membrane Protein Expression and Purification.Membrane Protein Solubilization and Stabilization.Manufacturability Assessment and Optimization.SIAT ® Immunogenicity Assessment Services.CreMap™ Epitope Mapping & Discovery Services.Dual-Polarization Interferometry (DPI) Services.Differential Scanning Calorimetry (DSC) Services.Isothermal Titration Calorimetry (ITC) Services.De Novo Human/Humanized Antibody Sequencing Service.Magic™ Human Antibody Production Using Transgenic Mice.Bovine Ultralong CDR3 Antibody Humanization.

Antibody Off-target Identification and Specificity Profiling Service.Biomolecular Interaction Analysis Services.Epitope Prediction for the Identification of Antibody Epitopes.Epitope Mapping by X-ray Crystallography.Phage Display & Antibody Library Services.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Īuthoritative and cutting-edge, Protein Scaffolds: Design, Synthesis, and Applications aims to ensure successful results in the study of this vital field. Site-specific bioconjugation methods, some unique protein architectures, and techniques that exploit peptide amphiphile micelles and assembly of chaperones are also featured. Chapters guide the readers through exploiting a number of protein scaffolds including virus nanoparticles, elastin and collagen peptides and proteins, and other protein templates for either building materials or presentation of ligands. This volume provides a wide range of methods and protocols detailing various protein structures as platforms for building architectures with targeted application.

Framework protein scaffold