#Protein scaffold heart Patch#

c-kit+ stem cells, improves cardiac repair 1, 2, 3, 4, and that mechanisms including paracrine effects, angiogenesis, transdifferentiation, and cell fusion have been suggested 2, 3, 5, 6. Accumulating experimental and clinical evidence indicates that intramyocardial transplantation of bone marrow (BM) undifferentiated cells, i.e. When ischemically impaired heart is unable to compensate for reduced cardiac output, heart failure results. Myocardial infarction (MI), also known as heart attack, leads to the loss of ischemic cardiomyocytes and degradation of myocardial extracellular matrices, characterized by left ventricular (LV) wall thinning and chamber dilation. Our in vitro and in vivo observations provide the first clinically relevant evidence for translating the hHVS-based biomaterials into clinical strategies to treat myocardial infarction. Thus, we have successfully developed a hHVS for cardiac repair.

#Protein scaffold heart Patch#

When used to patch infarcted heart in a murine model of myocardial infarction, either implantation of the hHVS alone or c-kit+ cell-seeded hHVS significantly improved cardiac function and reduced infarct size while c-kit+ cell-seeded hHVS was even superior to the hHVS alone. Upon anchoring onto the hHVS, post-infarct murine BM c-kit+ cells exhibited an increased capacity for proliferation and cardiomyogenic differentiation in vitro. In this study, human heart valve tissue was sliced into 100 μm tissue sheet by frozen-sectioning and then decellularized to form the hHVS. Here we hypothesized that human heart valve-derived scaffold (hHVS), as a clinically relevant novel biomaterial, may provide the proper microenvironment of native myocardial extracellular matrix for cardiac repair.

However, the bioactivity of biomaterials remains to be optimized because currently available biomaterials do not mimic the biochemical components as well as the structural properties of native myocardial extracellular matrix. Cardiac tissue engineering using biomaterials with or without combination of stem cell therapy offers a new option for repairing infarcted heart.